PhD position in Structural analysis of membrane protein complexes using cryo-EM

A PhD position is available in the lab "Structure and Function of Membrane Proteins” at the VIB-VUB Center for Structural Biology in Brussels, Belgium. The project focuses on the structural elucidation of membrane protein complexes and integrates fundamental cell biology and structural biology by cryo- electron microscopy.

The lab has a strong track record in investigating scramblases and their role in membrane remodeling. It also aims to deepen the mechanistic understanding of solute transport in ion channels and transporters of medical relevance.

We apply state of the art methods for the purification of fragile membrane protein complexes, either from cultured mammalian cells or from native sources. These complexes are studied through tailored biochemical, biophysical, and cell biological assays, including liposome preparations and confocal microscopy. For structural work, single particle cryo-electron microscopy is extensively utilized at the Biological Electron Cryogenic Microscopy Center of Flanders (BECM), Belgium’s prime location for cryo-EM, on our campus at VUB. The latest AI tools such as Alphafold 3 or BindCraft are integrated to propel research and accelerate discovery. Further details on the topic are discussed with the candidate during the interview phase.

Profile

Candidates must hold a Master’s in biochemistry, biophysics or related fields. A keen interest in eukaryotic cell biology, membrane proteins and structural biology is essential. The ability to work organized and independently with excellent communication skills is mandatory as well as proficiency in written and spoken English. Previous experience in mammalian cell culture, structural biology (cryo-EM) or membrane protein purification will be preferred. Candidates should demonstrate a strong motivation and commitment to solve scientific problems and a curiosity-driven approach in their work.

We offer

• Excellent opportunity for a challenging membrane protein structure project with translational aspects embedded in the core expertise of the team
• Extensive and excellent training for cryo-EM
• Dedicated new cell culture facility for adherent or suspension culture of mammalian cells
• State of the art systems for purification and analysis in vitro and in living cells
• The applicant will benefit from access to centralized facilities (in particular Nanobody technology, proteomics, cutting-edge microscopy and structural biology).

Please apply online with a cover letter detailing your background and interest in this position and your full CV including the contact information of three referees (with email, address and phone number). The position is available upon agreement. For further information visit https://brunnerlab.sites.vib.b... and contact Prof. Janine Brunner (janine.brunner@vub.be).